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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]
The mating of yeast, also known as yeast sexual reproduction, is a biological process that promotes genetic diversity and adaptation in yeast species. Yeast species, such as Saccharomyces cerevisiae (baker's yeast), are single-celled eukaryotes that can exist as either haploid cells, which contain a single set of chromosomes , or diploid cells ...
Another method of selection is the use of certain auxotrophic markers that can compensate for an inability to metabolise certain amino acids, nucleotides, or sugars. This method requires the use of suitably mutated strains that are deficient in the synthesis or utility of a particular biomolecule, and the transformed cells are cultured in a ...
Berberine, a plant compound traditionally used in herbal medicine, is today commonly stocked on the shelves of health food stores and pharmacies as a supplement. Beyond weight loss, berberine also ...
MOSCOW (Reuters) -Russia said on Wednesday that relations with Washington were so confrontational that Russian citizens should not visit the United States, Canada and some EU countries in coming ...
In the years following Hartwell's labor-intensive experiments, it has been shown that other environmental factors contribute to cellular fate in yeast and analogously in other organisms. Though not yeast-specific, a critical study put forth by Zetterberg et al . in 1985 provided evidence for a commitment point in Swiss 3T3 cells, or mouse ...
Agents on patrol discovered two backpacks stuffed with more than $1.1 million worth of cocaine in Washington state near the border with Canada, U.S. Customs and Border Protection said Monday. The ...