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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
In E. coli BL21(DE3) the expression of the T7-RNAP is suppressed by the constitutively expressed LacI repressor. LacI binds the lac operator, which is located downstream of the LacUV5 promoter, preventing the production of the T7-RNAP. However, upon supplementation of IPTG, the LacI repressor dissociates from the lac operator, allowing for the ...
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Index inducer or just inducer predictably induce metabolism via a given pathway and are commonly used in prospective clinical drug-drug interaction studies. [4] Strong, moderate, and weak inducers are drugs that decreases the AUC of sensitive index substrates of a given metabolic pathway by ≥80%, ≥50% to <80%, and ≥20% to <50% ...
Metaplasticity is a term describing the likelihood that a given stimulus will induce neuronal plasticity, based on the previous activity experienced by that neuron. Several studies provide evidence that neurons receiving “priming activity” (such as neurotransmitters, paracrine signals, or hormones) minutes to days prior will show a lower threshold for induction of long term potentiation (LTP).
Research suggests that using the Internet helps boost brain power for middle-aged and older people [17] (research on younger people has not been done). The study compares brain activity when the subjects were reading and when the subjects were surfing the Internet. It found that Internet surfing uses much more brain activity than reading does.
Bringing home a new baby is always a special time, and bringing one home during the holidays makes it even more special. Chase and Chester and Golden Retrievers that recently got a new baby sister ...
LTP could be produced by repetitive stimulation of the presynaptic terminals, and it is believed to play a role in memory function in the hippocampus, amygdala and other cortical brain structures in mammals. [2] [3] Long-term potentiation occurs when synaptic transmission becomes more effective as a result of recent activity.