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The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species: red colonies. Coliforms: yellow to orange colonies. Pseudomonas aeruginosa: pink, flat, rough colonies. This type of colony can be easily mistaken for Salmonella due to the color similarities. XLD agar contains:
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar ; however, the agar is supplemented with the surfactant, Tergitol 4 , which causes inhibition of Proteus spp. and other non-Salmonellae. [ 1 ]
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
The term is usually restricted to Shigella infections. [2] Shigellosis is caused by one of several types of Shigella bacteria. [3] Three species are associated with bacillary dysentery: Shigella sonnei, Shigella flexneri and Shigella dysenteriae. [4] A study in China indicated that Shigella flexneri 2a was the most common serotype. [5]
Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. Bismuth and brilliant green (dye) both inhibit gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide. Bismuth sulfite agar typically contains : [1]
Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria .
Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar . References
Salmonella spp appear to be yellow or colourless colonies, often with a dark centre. As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be ...