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samtools sort -o sorted_out unsorted_in.bam. Read the specified unsorted_in.bam as input, sort it by aligned read position, and write it out to sorted_out. Type of output can be either sam, bam, or cram, and will be determined automatically by sorted_out's file-extension. samtools sort -m 5000000 unsorted_in.bam sorted_out
The SAM format consists of a header and an alignment section. [1] The binary equivalent of a SAM file is a Binary Alignment Map (BAM) file, which stores the same data in a compressed binary representation. [4] SAM files can be analysed and edited with the software SAMtools. [1] The header section must be prior to the alignment section if it is ...
Binary Alignment Map (BAM) is the comprehensive raw data of genome sequencing; [1] it consists of the lossless, compressed binary representation of the Sequence Alignment Map-files. [2] [3] BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments. [4]
It takes SAM/BAM/CRAM or kallisto abundance files directly as input, and produces fold-changes and exact P-values for up to 5 replicates, near-exact P-values for up to 12 replicates, and Mann-Whitney (Wilcoxon rank-sum) P-values for 8 or more replicates. featureCounts an efficient general-purpose read quantifier. FDM
The SAM/BAM files use the CIGAR (Compact Idiosyncratic Gapped Alignment Report) string format to represent an alignment of a sequence to a reference by encoding a sequence of events (e.g. match/mismatch, insertions, deletions).
The read alignments are sorted using SAMtools, after which variant callers such as GATK [20] are used to identify differences compared to the reference sequence. The choice of variant calling tool depends heavily on the sequencing technology used, so GATK is often used when working with short reads, while long read sequences require tools like ...
The Variant Call Format or VCF is a standard text file format used in bioinformatics for storing gene sequence or DNA sequence variations. The format was developed in 2010 for the 1000 Genomes Project and has since been used by other large-scale genotyping and DNA sequencing projects.
SAMtools: Utilities for interacting with high-throughput sequencing data and alignments in sam/bam format Unix/Linux: MIT: Collaborative project SOAP Suite: Suite of tools for assembly, alignment, and analysis of short read next generation sequencing data Unix/Linux, macOS: GPL: BGI: Staden Package