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Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA...
RNA-Seq has a 5-log dynamic range, providing exceptional relative quantitation of transcripts and making detection of rare transcripts possible. A wide variety of RNA types can be sequenced, including polyadenylated [polyA(+)] and non-polyadenylated mRNAs, non-coding RNAs, and microRNAs.
Bulk RNA sequencing is the method of choice for transcriptomic analysis of pooled cell populations, tissue sections, or biopsies. It measures the average expression level of individual genes across hundreds to millions of input cells and is useful to get a global idea of gene expression differences between samples.
Herein, we introduce Bulk2Space, a spatial deconvolution algorithm based on deep learning frameworks, which generates spatially resolved single-cell expression profiles from bulk transcriptomes...
Kallisto, bustools and kb-python are a set of tools for quantifying bulk, single-cell and single-nucleus RNA-seq. Together, this set of free, open-source software tools can produce gene...
Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs.
Practical RNA sequencing (RNA-Seq) applications have evolved in conjunction with sequence technology and bioinformatic tools advances. In most projects, bulk RNA-Seq data is used to measure gene expression patterns, isoform expression, alternative splicing and single-nucleotide polymorphisms.
Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing.
Our goals in the present review are to break down the steps of a typical RNA-seq analysis and to highlight the pitfalls and checkpoints along the way that are vital for bench scientists and biomedical researchers performing experiments that use RNA-seq.
Bulk RNA-seq, or bulk RNA sequencing, allows researchers to analyze the entire transcriptome of a sample, providing valuable insights into the types and quantities of RNA molecules present.