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The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2]
Then all the frozen tissue cores are inserted in a recipient OCT frozen block in a precisely spaced, array pattern. Sections from this block are cut using a cryostat, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each frozen tissue array block can be cut into 100–500 sections, which can be ...
The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...
Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.
Frozen section procedure: water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome-cryostat; sections are stained and examined with a light microscope. This technique is much faster than traditional histology (5 minutes vs 16 hours) and is used in conjunction with medical procedures to ...
In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component. [1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes.
Advanced Placement (AP) Biology (also known as AP Bio) is an Advanced Placement biology course and exam offered by the College Board in the United States. For the 2012–2013 school year, the College Board unveiled a new curriculum with a greater focus on "scientific practices".
Each microarray block can be cut into 100 – 500 sections, which can be subjected to independent tests. Tests commonly employed in tissue microarray include immunohistochemistry, and fluorescent in situ hybridization. Tissue microarrays are particularly useful in analysis of cancer samples. One variation is a Frozen tissue array.