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  2. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The lifespan of most cells is genetically determined, but some cell-culturing cells have been 'transformed' into immortal cells which will reproduce indefinitely if the optimal conditions are provided.

  3. Tissue culture - Wikipedia

    en.wikipedia.org/wiki/Tissue_culture

    Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. [1] This is possible only in certain conditions. It also requires more attention.

  4. Cell-based vaccine - Wikipedia

    en.wikipedia.org/wiki/Cell-based_vaccine

    Cell lines grown in synthetic media avoid animal serum, which may pose a sterility problem, more specifically, preventing the spread of transmissible spongiform encephalopathies. [9] [10] [11] Another benefit is the avoidance of egg-allergen. Lastly, cell-based vaccines may be more effective given that, with egg-based vaccines, there is a risk ...

  5. Hybridoma technology - Wikipedia

    en.wikipedia.org/wiki/Hybridoma_technology

    (4) Fusion of myeloma and B cells (5) Separation of cell lines (6) Screening of suitable cell lines (7) in vitro (a) or in vivo (b) multiplication (8) Excised spleen processed for antibody extraction Hybridoma cells grown in tissue culture. The image shows a single clone of cells each of which is producing large amounts of a specific monoclonal ...

  6. Suspension culture - Wikipedia

    en.wikipedia.org/wiki/Suspension_culture

    SH-SY5Y cells adhered to a surface. The history of suspension cell culture is closely tied to the overall history of cell and tissue culture. In 1885, Wilhelm Roux laid the groundwork for future tissue culture, by developing a saline buffer that was used to maintain living cells (chicken embryos) for a few days. [6]

  7. Stem-cell line - Wikipedia

    en.wikipedia.org/wiki/Stem-cell_line

    A stem cell line is a group of stem cells that is cultured in vitro and can be propagated indefinitely. Stem cell lines are derived from either animal or human tissues and come from one of three sources: embryonic stem cells, adult stem cells, or induced pluripotent stem cells. They are commonly used in research and regenerative medicine.

  8. HEK 293 cells - Wikipedia

    en.wikipedia.org/wiki/HEK_293_cells

    Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [ 1 ] [ 2 ] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection .

  9. Trypsinization - Wikipedia

    en.wikipedia.org/wiki/Trypsinization

    This process of cell culture or tissue culture requires a method to dissociate the cells from the container and each other. Trypsin, an enzyme commonly found in the digestive tract , can be used to "digest" the proteins that facilitate adhesion to the container and between cells.

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