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Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
Abbe number. In optics and lens design, the Abbe number, also known as the V-number or constringence of a transparent material, is an approximate measure of the material's dispersion (change of refractive index versus wavelength), with high values of V indicating low dispersion. It is named after Ernst Abbe (1840–1905), the German physicist ...
In optics, the Abbe sine condition is a condition that must be fulfilled by a lens or other optical system in order for it to produce sharp images of off-axis as well as on-axis objects. It was formulated by Ernst Abbe in the context of microscopes. [ 1] The Abbe sine condition says that. the sine of the object-space angle should be ...
Super-resolution microscopy. Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, [ 1][ 2] which is due to the diffraction of light. [ 3] Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy ...
The polio virus is 30 nm in diameter. [ 1] Transmission electron microscopy ( TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons ...
Condenser (optics) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds ...
A superlens, or super lens, is a lens which uses metamaterials to go beyond the diffraction limit. The diffraction limit is a feature of conventional lenses and microscopes that limits the fineness of their resolution depending on the illumination wavelength and the numerical aperture (NA) of the objective lens.
STED microscopy is one of several types of super resolution microscopy techniques that have recently been developed to bypass the diffraction limit of light microscopy to increase resolution. STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to ...