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A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the rearrangement is present. Fluorescence in situ hybridization ( FISH) is a molecular cytogenetic ...
A bioassay is a biochemical test to estimate the potency of a sample compound. Usually this potency can only be measured relative to a standard compound. [ 3][ 1] A typical bioassay involves a stimulus (ex. drugs) applied to a subject (ex. animals, tissues, plants). The corresponding response (ex. death) of the subject is thereby triggered and ...
Each local IACUC reviews research protocols and conducts evaluations of the institution's animal care and use, which includes the results of inspections of facilities that are required by law. [4] The corresponding, parallel, and equivalent local ethical body responsible for overseeing U.S. federally funded research involving humans is the ...
In the case of TRF assays, a wash step is required to remove unbound fluorescent reagents prior to measuring the activity signal of the assay. This increases reagent use, time to complete the assay, and limits the ability to miniaturize the system (e.g. converting from a 384-well microtiter plate to a 1536-well plate). [5]
First, a new dog breed must meet a list of criteria, such as a recorded 3-generation pedigree and a minimum population. Then, they will submit their breed information to the AKC Board of Directors ...
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The dog wouldn't stop watching them — even when they got to the end of the block. People in the comments section also thought this was a betrayal. "She said 'you’re taking her to OUR spots ...
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) [ 1] and protein–DNA interactions [ 2][ 3] by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.
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