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Simmons’ citrate agar was developed by James S. Simmons in 1926 by adding 1.5% agar and bromothymol blue as a pH indicator to Koser’s citrate agar to observe changes in pH as a result of oxidative reactions from citrate metabolism. [5]
Use of citrate involves the enzyme citrate lyase, which breaks down citrate to oxaloacetate and acetate. Oxaloacetate is further broken down to pyruvate and carbon dioxide (CO 2). Production of sodium bicarbonate (NaHCO 3) as well as ammonia (NH 3) from the use of sodium citrate and ammonium salts results in alkaline pH. This results in a ...
The agar contains citrate and ammonium ions (nitrogen source) and bromothymol blue (BTB) as a pH indicator. [2] Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized.
DCA agar (deoxycholate citrate agar) is a solid bacteriological growth medium used for isolation of enteric pathogens. [1] Uses
Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar.
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
Thiosulfate–citrate–bile salts–sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. [1] TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS agar, other rapid testing ...