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  2. Simmons' citrate agar - Wikipedia

    en.wikipedia.org/wiki/Simmons'_citrate_agar

    Although it could be used in other formats (e.g., Petri plates), Simmons' citrate agar is often used in slants or slopes in test tubes. One advantage of using slants over Petri dishes is access to oxygen, which is required by citrate metabolism. Simmons’ agar can be bought from suppliers as ready-made powders or slants.

  3. TSI slant - Wikipedia

    en.wikipedia.org/wiki/TSI_slant

    The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.

  4. Inoculation needle - Wikipedia

    en.wikipedia.org/wiki/Inoculation_needle

    The slant culture cap is then removed and secured using the needle hand. Flaming the open end of the slant culture will prevent contamination and the formation of aerosols. [2] [3] Transfer happens once the tip of the inoculation needle comes into contact with the agar surface of the slant culture. The inoculation needle should not move in the ...

  5. IMViC - Wikipedia

    en.wikipedia.org/wiki/IMViC

    The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized. The test is also prepared on a slant to maximize bacterial growth for an even better indication of the use of citrate.

  6. Löwenstein–Jensen medium - Wikipedia

    en.wikipedia.org/wiki/Löwenstein–Jensen_medium

    Sodium citrate; The original formulation included starch, which was later found to be unnecessary, so omitted. Low levels of penicillin and nalidixic acid are also present in LJ medium to inhibit growth of Gram-positive and Gram-negative bacteria, to limit growth to Mycobacterium species only. Presence of malachite green in the medium inhibits ...

  7. Lysine iron agar - Wikipedia

    en.wikipedia.org/wiki/Lysine_iron_agar

    A frequent test done with LIA agar is the LIA slant. Here the LIA is solidified at an angle, then inoculated with bacteria by stabbing the agar to within 1/4 inch of the bottom of the tube and streaking the slant. The slant is then incubated at 35 °C for 18–24 hours. The results are scored as follows: purple slant/yellow butt = LSI Negative

  8. Mueller–Hinton agar - Wikipedia

    en.wikipedia.org/wiki/Mueller–Hinton_agar

    Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton , who were microbiologists working at Harvard University.

  9. Apocholate citrate agar - Wikipedia

    en.wikipedia.org/wiki/Apocholate_citrate_agar

    Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar.