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The reverse CAMP test is a method to identify Clostridium perfringens using β-hemolytic streptococci. The CAMP factor produced by S. agalactiae and the alpha toxin produced by C. perfringens act synergistically to produce enhanced hemolysis. Streaking these two organisms perpendicular to each other on a blood agar plate will yield a “bow tie ...
The reverse CAMP test utilizes the synergetic hemolytic abilities of the CAMP factor produced by Streptococcus agalactiae with the α-toxin produced by Clostridium perfringens. Streaking these two organisms perpendicular to each other on a blood agar plate will yield a “bow-tie” clearing of the blood agar by the hemolytic capabilities of ...
This phenomenon is the mechanism behind the CAMP test [2], a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes. [3] A modified version of this test, utilizing S. agalactiae instead of S. aureus, called the reverse CAMP test can also be used to identify Clostridium perfringens.
Clostridium perfringens (formerly known as C. welchii, or Bacillus welchii) is a Gram-positive, bacillus (rod-shaped), anaerobic, spore-forming pathogenic bacterium of the genus Clostridium. [ 1 ][ 2 ]C. perfringens is ever-present in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of ...
Colonial morphology. In microbiology, colonial morphology refers to the visual appearance of bacterial or fungal colonies on an agar plate. Examining colonial morphology is the first step in the identification of an unknown microbe. The systematic assessment of the colonies' appearance, focusing on aspects like size, shape, colour, opacity, and ...
Voges–Proskauer / ˈfoʊɡəs ˈprɒskaʊ.ər / or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a glucose-phosphate broth that has been inoculated with bacteria. A cherry red color indicates a positive result ...
In microbiology, the minimum inhibitory concentration (MIC) is the lowest concentration of a chemical, usually a drug, which prevents visible in vitro growth of bacteria or fungi. [ 1 ][ 2 ] MIC testing is performed in both diagnostic [ 1 ][ 2 ] and drug discovery laboratories. [ 3 ][ 4 ] The MIC is determined by preparing a dilution series of ...
Durham tubes are used in microbiology to detect production of gas by microorganisms. They are simply smaller test tubes inserted upside down in another test tube so they are freely movable. The culture media to be tested is then added to the larger tube and sterilized, which also eliminates the initial air gap produced when the tube is inserted ...