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The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. [2] [3] Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks.
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]
CHO cells in suspension. A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The ...
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate-derived cells (with the exception of hematopoietic cells) are anchorage dependent and require a two-dimensional monolayer to facilitate cell adhesion, spreading and replication. [2]
For adherent cells, cell density is normally measured in terms of confluency, the percentage of the growth surface covered by cells. The cells will often have a known range of confluencies for optimal growth, for example a mammalian cell line like HeLa generally prefers confluencies between 10% and 100%, and subculture will normally try to keep ...
Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel.
Thus, on the one hand the system provides a highly adaptable basis for the culture of adherent cells and the generation of specialized tissues. On the other hand the Minusheet perfusion culture system is bridging a methodical gap between the conventional static 24 well culture plate and modern perfusion culture technology.
Adherent cells require a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix (such as collagen and laminin) components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent.