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Golden Gate assembly involves digesting DNA sequences containing a type IIS restriction enzyme cut site and ligating them together. Golden Gate Cloning or Golden Gate assembly [1] is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase. [2]
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Troubleshooting is a form of problem solving, often applied to repair failed products or processes on a machine or a system. It is a logical, systematic search for the source of a problem in order to solve it, and make the product or process operational again. Troubleshooting is needed to identify the symptoms.
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The seven basic tools of quality are a fixed set of visual exercises identified as being most helpful in troubleshooting issues related to quality. [1] They are called basic because they are suitable for people with little formal training in statistics and because they can be used to solve the vast majority of quality-related issues. [2]
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The rivet assembly is inserted into a hole drilled through the parts to be joined and a specially designed tool is used to draw the mandrel through the rivet. The compression force between the head of the mandrel and the tool expands the diameter of the tube throughout its length, locking the sheets being fastened if the hole was the correct size.
The rat's nest is a jumble of wires (lines) criss-crossing each other to their destination nodes. These wires are routed either manually or automatically by the use of electronics design automation (EDA) tools. The EDA tools arrange and rearrange the placement of components and find paths for tracks to connect various nodes.