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  2. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    After its introduction in the 1940s, live-cell imaging rapidly became popular using phase-contrast microscopy. [11] The phase-contrast microscope was popularized through a series of time-lapse movies (see video), recorded using a photographic film camera. [12] Its inventor, Frits Zernike, was awarded the Nobel Prize in 1953. [13]

  3. Oil immersion - Wikipedia

    en.wikipedia.org/wiki/Oil_immersion

    The resolution of a microscope is defined as the minimum separation needed between two objects under examination in order for the microscope to discern them as separate objects. This minimum distance is labelled δ. If two objects are separated by a distance shorter than δ, then they will appear as a single object in the microscope.

  4. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...

  5. Microscopy with UV surface excitation - Wikipedia

    en.wikipedia.org/wiki/Microscopy_with_UV_surface...

    The microscope setup is based on an inverted microscope design. [ 2 ] [ 3 ] [ 4 ] An automated stage is used to record larger areas by mosaicing a series of single adjacent frames. The LED light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy ...

  6. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    Scientist using an optical microscope in a laboratory. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present ...

  7. Instrument myopia - Wikipedia

    en.wikipedia.org/wiki/Instrument_myopia

    When someone looks into an optical instrument, such as a microscope, vision is far from ordinary. A microscope might force the person to use only one eye, it presents the person with a limited field of view, it presents a magnified view, and it allows the person to adjust the focus of the instrument for any viewing distance. Ideally, the person ...

  8. Micrograph - Wikipedia

    en.wikipedia.org/wiki/Micrograph

    This is opposed to a macrograph or photomacrograph, an image which is also taken on a microscope but is only slightly magnified, usually less than 10 times. Micrography is the practice or art of using microscopes to make photographs. A photographic micrograph is a photomicrograph, and one taken with an electron microscope is an electron micrograph.

  9. Photoemission electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Photoemission_electron...

    By using an energy filter, a PEEM microscope can be seen as imaging Ultra-violet photoelectron spectroscopy (UPS) or X-ray photoelectron spectroscopy (XPS). By using this method, spatially resolved photoemission spectra can be acquired with spatial resolutions on the 100 nm scale and with sub-eV resolution.