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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
A variety of templates and styles are available to create timelines. The {{Graphical timeline}} template allows representations of extensive timelines. The template offers complex formatting and labeling options to control the output. Typically, each use is made into its own template, and the template is then transcluded into the article.
{{Graph, chart and plot templates | state = collapsed}} will show the template collapsed, i.e. hidden apart from its title bar. {{ Graph, chart and plot templates | state = autocollapse }} will show the template autocollapsed, i.e. if there is another collapsible item on the page (a navbox, sidebar , or table with the collapsible attribute ...
Pyrosequencing uses luciferase to generate light for detection of the individual nucleotides added to the nascent DNA, and the combined data are used to generate sequence reads. [81] This technology provides intermediate read length and price per base compared to Sanger sequencing on one end and Solexa and SOLiD on the other. [90]
English: Gray, blue, red, green, light green, black graph papers with 1 cm–0.5 cm–1 mm grids (page size: A4) in printable PDF format. Date 25 July 2013, 18:04:17
This page has needed work for a while. I'll get around to helping out one of these days. Spin2cool 18:32, 4 May 2007 (UTC) After reading the introduction I feel that the 454 should only be mentioned later under recent advantages. This way it appears that 454 is the only way pyrosequencing is applied. In addition, it is a bit of a repetition.
In a de Bruijn graph, there is a possibility of 4^k different nodes to make arrangements of a genome. The number of nodes used to create the graph can be reduced in number by considering only the k-mers found within the DNA strand of interest. Given sequence 1, it is possible to determine the nodes of size 7, or 7-mers, that will be in the graph.