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Biophysical chemistry is a physical science that uses the concepts of physics and physical chemistry for the study of biological systems. [1] The most common feature of the research in this subject is to seek an explanation of the various phenomena in biological systems in terms of either the molecules that make up the system or the supra-molecular structure of these systems. [2]
Circular Dichroism – method to measure chirality of a sample using circularly polarized light. This technique is commonly used to determine protein structure. Computational chemistry – use of numerical methods to probe the structure and dynamical equilibrium in biological systems. Cryobiology – studies the effects of low temperatures on ...
Physical biochemistry is a branch of biochemistry that deals with the theory, techniques, and methodology used to study the physical chemistry of biomolecules. [1] It also deals with the mathematical approaches for the analysis of biochemical reaction and the modelling of biological systems .
Biogeochemistry is the scientific discipline that involves the study of the chemical, physical, geological, and biological processes and reactions that govern the composition of the natural environment (including the biosphere, the cryosphere, the hydrosphere, the pedosphere, the atmosphere, and the lithosphere).
Phenol: The phenol used for biochemistry comes as a water-saturated solution with Tris buffer, as a Tris-buffered 50% phenol, 50% chloroform solution, or as a Tris-buffered 50% phenol, 48% chloroform, 2% isoamyl alcohol solution (sometimes called "25:24:1"). Phenol is naturally somewhat water-soluble, and gives a fuzzy interface, which is ...
In these methods, a reacting system initially at equilibrium is perturbed rapidly and then observed as it relaxes back to equilibrium. [ 1 ] [ 2 ] [ 3 ] In the case of temperature jump, the perturbation involves rapid heating which changes the value of the equilibrium constant , followed by relaxation to equilibrium at the new temperature.
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.
A ribosome is a biological machine that utilizes protein dynamics. Molecular biophysics is a rapidly evolving interdisciplinary area of research that combines concepts in physics, chemistry, engineering, mathematics and biology. [1]