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  2. Restriction fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Restriction_fragment...

    A restriction fragment length polymorphism is said to occur when the length of a detected fragment varies between individuals, indicating non-identical sequence homologies. Each fragment length is considered an allele, whether it actually contains a coding region or not, and can be used in subsequent genetic analysis.

  3. Terminal restriction fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Terminal_restriction...

    Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene.

  4. Restriction site associated DNA markers - Wikipedia

    en.wikipedia.org/wiki/Restriction_site...

    The density of RAD tags in a genome depends on the restriction enzyme used during the isolation process. [5] There are other restriction site marker techniques, like RFLP or amplified fragment length polymorphism (AFLP), which use fragment length polymorphism caused by different restriction sites, for the distinction of genetic polymorphism ...

  5. Amplified fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Amplified_fragment_length...

    Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Pieter Vos, [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...

  6. Genetic marker - Wikipedia

    en.wikipedia.org/wiki/Genetic_marker

    RFLP (or Restriction fragment length polymorphism) SSLP (or Simple sequence length polymorphism) AFLP (or Amplified fragment length polymorphism) RAPD (or Random amplification of polymorphic DNA) VNTR (or Variable number tandem repeat) SSCP (or Single-strand conformation polymorphism) SSR Microsatellite polymorphism, (or Simple sequence repeat) [2]

  7. SNP genotyping - Wikipedia

    en.wikipedia.org/wiki/SNP_genotyping

    Restriction fragment length polymorphism (RFLP) is considered to be the simplest and earliest method to detect SNPs. SNP-RFLP makes use of the many different restriction endonucleases and their high affinity to unique and specific restriction sites.

  8. Restriction site - Wikipedia

    en.wikipedia.org/wiki/Restriction_site

    Restriction sites can be used for multiple applications in molecular biology such as identifying restriction fragment length polymorphisms . Restriction sites are also important consideration to be aware of when designing plasmids.

  9. Forensic DNA analysis - Wikipedia

    en.wikipedia.org/wiki/Forensic_DNA_analysis

    The first true method of DNA profiling was restriction fragment length polymorphism analysis. The first use of RFLP analysis in forensic casework was in 1985 in the United Kingdom. [4] This type of analysis used variable number tandem repeats (VNTRs) to distinguish between individuals.