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It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures to be stained very well by basic dyes. This can be explained by their ...
Unlike negative staining, positive staining uses basic dyes to color the specimen against a bright background. While chromophore is used for both negative and positive staining alike, the type of chromophore used in this technique is a positively charged ion instead of a negative one.
Download as PDF; Printable version; ... Staining dyes are dyes used for staining in microbiology and histology.
Basic dyes are also used in the coloration of paper. Direct or substantive dyeing is normally carried out in a neutral or slightly alkaline dye bath, at or near boiling point, with the addition of either sodium chloride (NaCl) or sodium sulfate (Na 2 SO 4) or sodium carbonate (Na 2 CO 3). Direct dyes are used on cotton, paper, leather, wool ...
Trypan Blue, a living-cell exclusion dye; Triphenyl tetrazolium chloride; Erythrosine, which is Red No. 3 in food coloring, can be used as an exclusion dye. 7-Aminoactinomycin D used e.g. in flowcytometric studies of hematopoietic stem cell viability. Other Vital Stains : Janus Green B is a basic dye and vital stain used in histology.
Bismarck brown Y also called C.I. 21000 [1] and C.I. Basic Brown 1, [1] is a diazo dye with the idealized formula [(H 2 N) 2 C 6 H 3 N 2] 2 C 6 H 4. The dye is a mixture of closely related compounds. It was one of the earliest azo dyes, being described in 1863 by German chemist Carl Alexander von Martius. It is used in histology for staining ...
It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing concentrations of ethanol. Lastly, back into water.
Adjective dyes are those dyes that require use of a mordant to bind the color to the fiber. [2] alkanet Alkanet or dyer's bugloss (Alkanna tinctoria) is a traditional plant source of red dye. [3] alum Alum (aluminum sulfate) is a naturally occurring basic mordant widely used in the ancient world. [4] añil