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The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
A cryostat (from cryo meaning cold and stat meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium . [ 1 ]
The cryonics procedure performed by the Cryonics Institute begins with a process called vitrification where the body is perfused with cryoprotective agents to protect against damage in the freezing process. After this, the body is cooled to -196°C over a day or two days in a computer-controlled chamber before being placed in a long-term ...
Dermatologic surgical procedures are treatments aimed at managing a wide range of medically necessary and cosmetic conditions, with a long history dating back to ancient times. Medically necessary dermatologic surgical procedures include curettage and electrosurgery , and Mohs surgery for the treatment of skin cancer, as well as skin grafting ...
Decontamination of humans is usually done by a three-step procedure, separated by sex: removal of clothing, washing, and reclothing. People suspected of being contaminated are usually separated by sex, and led into a decontamination tent, trailer, or pod, where they shed their potentially contaminated clothes in a strip-down room.
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Sections from this block are cut using a cryostat, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each frozen tissue array block can be cut into 100–500 sections, which can be subjected to independent tests.
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