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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
A counting chamber. A counting chamber, is a microscope slide that is especially designed to enable cell counting. Hemocytometers and Sedgewick Rafter counting chambers are two types of counting chambers. The hemocytometer has two gridded chambers in its middle, which are covered with a special glass slide when counting.
Cytometers are the instruments which count the blood cells in the common blood test.. Cytometry is the measurement of number and characteristics of cells.Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the ...
Previously, this procedure involved preparing a peripheral blood smear and manually counting each type of cell under a microscope, a process that typically required a half-hour. A Coulter counter played an important role in the development of the first cell sorter , and was involved in the early development of flow cytometry .
Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. [5] Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: Cell counting; Cell sorting; Determining cell characteristics and function; Detecting microorganisms; Biomarker ...
When the method only recounts living organisms is called "viable count". [2] There are many methods for the quantification of microorganisms, including microscopy methods, Coulter counter, Mass Spectrometry (for estimating cell mass), and Cell Culture methods which form and grow colonies of bacteria.
Counting of cells: positive responder cells are count from the lower chamber (long incubation time) or from the filter (short incubation time). For detection of cells general staining techniques (e.g. trypan blue ) or special probes (e.g. mt-dehydrogenase detection with MTT assay) are used.
Anaerobic bacteria collection can come from a variety of sources in patient samples, including blood, bile, bone marrow, cerebrospinal fluid, direct lung aspirate, tissue biopsies from a normally sterile site, fluid from a normally sterile site (like a joint), dental, abscess, abdominal or pelvic abscess, knife, gunshot, or surgical wound, or ...