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This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the ...
Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
Gail Roberta Martin (née Zuckman, born 1944) is an American biologist. She is professor emerita in the Department of Anatomy, University of California, San Francisco.She is known for her pioneering work on the isolation of pluripotent stem cells from normal embryos, for which she coined the term 'embryonic stem cells'. [1]
MRC-5 cell. MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted Caucasian male fetus.
Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmissions, MEFs will senesce and finally die off.
100532731 12151 Ensembl ENSG00000269897 ENSMUSG00000026739 UniProt P35226 P25916 RefSeq (mRNA) NM_001204062 NM_007552 RefSeq (protein) NP_001190991 NP_005171 NP_031578 Location (UCSC) Chr 10: 22.32 – 22.33 Mb Chr 2: 18.68 – 18.69 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse The COMMD3-BMI1 gene in humans encodes the COMMD3-BMI1 readthrough protein. Function This locus ...
Cell Culture Applications - Resources including application notes and protocols to create an ideal environment for growing cells, right from the start. Cell Culture Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique including basic cell culture protocols and video training
Isolation from a naturally occurring cancer. This is the original method for generating an immortalised cell line. A major example is human HeLa , a line derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951.
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