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The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Another common presumptive test is the Kastle-Meyer or Phenolphthalein test. This is a catalytic test that detects the heme group in blood that transports oxygen and carbon dioxide. [ 5 ] A sterile cotton swab is soaked in distilled water and applied to the area of suspected blood to pick up some of the sample. [ 5 ]
Most people who take a drug test take a presumptive test, cheaper and faster than other methods of testing. However, it is less accurate and can render false results. The FDA recommends for confirmatory testing to be conducted and the placing of a warning label on the presumptive drug test: "This assay provides only a preliminary result.
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The Kastle Meyer (KM) reagent is actually already basic in pH -- it is when the reduced phenolphthalein in the reagent is oxidized to phenolphthalein by the presence of heme (or some other oxidizer) that it is the phenolphthalein you are familiar with, which indeed, will turn pink in the presence of its alkaline solvent.
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