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The term matrix-assisted laser desorption ionization (MALDI) was coined in 1985 by Franz Hillenkamp, Michael Karas and their colleagues. [3] These researchers found that the amino acid alanine could be ionized more easily if it was mixed with the amino acid tryptophan and irradiated with a pulsed 266 nm laser.
MALDI mass spectrometry imaging (MALDI-MSI) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging [2] technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. [3]
MALDESI source can also be coupled to drift tube ion mobility spectrometer-mass spectrometer (IMS-MS) for high-throughput screening. [15] These integrations provide more reliable raw data for MSI as well as direct analysis of various biological specimens because long analysis time could cause physiological changes to the samples under ...
Mascot performs mass spectrometry data analysis through a statistical evaluation of matches between observed and projected peptide fragments. [6] MassMatrix: Freeware: MassMatrix is a database search algorithm for tandem mass spectrometric data. It uses a mass accuracy-sensitive, probabilistic scoring model to rank peptide and protein matches ...
A Bradbury–Nielsen shutter is a type of ion gate used in TOF mass spectrometers and in ion mobility spectrometers, as well as Hadamard transform TOF mass spectrometers. [9] The Bradbury–Nielsen shutter is ideal for fast timed ion selector (TIS)—a device used for isolating ions over narrow mass range in tandem (TOF/TOF) MALDI mass ...
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
In SEAC, the sample surface is modified to bind the analyte of interest for analysis with laser desorption/ionization mass spectrometry (LDI-MS). [ 1 ] [ 4 ] [ 6 ] SEPAR is a combination of SEND and SEAC; the modified sample surface also acts as an energy absorbing matrix for ionization.
In mass spectrometry, a matrix is a compound that promotes the formation of ions. Matrix compounds are used in matrix-assisted laser desorption/ionization (MALDI), matrix-assisted ionization (MAI), and fast atom bombardment (FAB).