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Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
Chemical structure of 2-bromophenol. A bromophenol is an organic compound consisting of hydroxyl groups and bromine atoms bonded to a benzene ring. They may be viewed as hydroxyl derivatives of bromobenzene, or as brominated derivatives of phenol. There are five basic types of bromophenols (mono- to pentabromophenol) and 19 different ...
Close-up of DNA ladders on an agarose gel. GelRed stain was used. Loading of a sample into a polyacrylamide gel electrophoresis well. An electrophoretic color marker is a chemical used to monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) since DNA, RNA, and most proteins are colourless. [1]
2-Bromophenol: 3-Bromophenol: 4-Bromophenol: Other names o-Bromophenol: m-Bromophenol: p-Bromophenol Chemical structure: CAS number: 95-56-7: 591-20-8: 106-41-2 PubChem ID CID 7244 from PubChem: CID 11563 from PubChem: CID 7808 from PubChem: Chemical formula: C 6 H 5 BrO Molar mass: 173.02 g/mol 1: Physical state: liquid solid Melting point: 3 ...
The extent of dichromatism of material can be quantified by the Kreft's dichromaticity index (DI). It is defined as the difference in hue angle (Δh ab) between the colour of the sample at the dilution, where the chroma (colour saturation) is maximal and the colour of four times more diluted (or thinner) and four times more concentrated (or thicker) sample.
Bromothymol blue acts as a weak acid in a solution. It can thus be in protonated or deprotonated form, appearing yellow or blue, respectively. It is bright aquamarine by itself, and greenish-blue in a neutral solution. The deprotonation of the neutral form results in a highly conjugated structure, accounting for the difference in color. An ...
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Xylene cyanol and Bromophenol blue are common dyes found in loading buffers; they run about the same speed as DNA fragments that are 5000 bp and 300 bp in length respectively, but the precise position varies with percentage of the gel.