Search results
Results from the WOW.Com Content Network
Conversely, when pH = pK a, the concentration of HA is equal to the concentration of A −. The buffer region extends over the approximate range pK a ± 2. Buffering is weak outside the range pK a ± 1. At pH ≤ pK a − 2 the substance is said to be fully protonated and at pH ≥ pK a + 2 it is fully dissociated (deprotonated).
The Henderson–Hasselbalch equation can be used to model these equilibria. It is important to maintain this pH of 7.4 to ensure enzymes are able to work optimally. [10] Life threatening Acidosis (a low blood pH resulting in nausea, headaches, and even coma, and convulsions) is due to a lack of functioning of enzymes at a low pH. [10]
Since Tris' pKa is more strongly temperature dependent, its use is not recommended in biochemical applications requiring consistent pH over a range of temperatures. Moreover, the temperature dependance of the pKa (and in turn buffer solution pH) makes pH adjustment difficult. [8] (E.g., the 'room temperature' pH adjustment would not translate ...
The dissociation constant for a particular ligand–protein interaction can change with solution conditions (e.g., temperature, pH and salt concentration). The effect of different solution conditions is to effectively modify the strength of any intermolecular interactions holding a particular ligand–protein complex together
The buffering region is dependent upon the pKa, and is typically +/- 1.0 pH units of the pKa. The pKa of KHP is 5.4, so its pH buffering range would be 4.4 to 6.4; however, due to the presence of the second acidic group that bears the potassium ion, the first pKa also contributes to the buffering range well below pH 4.0, which is why KHP is a ...
The neutral value of the pH depends on the temperature and is lower than 7 if the temperature increases above 25 °C. The pH range is commonly given as zero to 14, but a pH value can be less than 0 for very concentrated strong acids or greater than 14 for very concentrated strong bases. [2] The pH scale is traceable to a set of standard ...
In aqueous solution, ammonia deprotonates a small fraction of the water to give ammonium and hydroxide according to the following equilibrium: . NH 3 + H 2 O ⇌ NH + 4 + OH −.. In a 1 M ammonia solution, about 0.42% of the ammonia is converted to ammonium, equivalent to pH = 11.63 because [NH +
Chemical formula. C 8 H 10 N 4 O 2: Molar mass: 194.194 g·mol −1 Appearance ... Except where noted otherwise, data relate to Standard temperature and pressure.