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Zinc finger nucleases have also been used in a mouse model of haemophilia [31] and a clinical trial found CD4+ human T-cells with the CCR5 gene disrupted by zinc finger nucleases to be safe as a potential treatment for HIV/AIDS. [32] ZFNs are also used to create a new generation of genetic disease models called isogenic human disease models.
In addition, zinc fingers have become extremely useful in various therapeutic and research capacities. Engineering zinc fingers to have an affinity for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications of this to be realized to date.
Gene therapy is a medical technology that aims to produce a therapeutic effect ... using techniques such as zinc finger nucleases and ... During the COVID-19 ...
Zinc finger protein chimera are chimeric proteins composed of a DNA-binding zinc finger protein domain and another domain through which the protein exerts its effect. The effector domain may be a transcriptional activator (A) or repressor (R), [1] a methylation domain (M) or a nuclease (N).
Zinc finger antiviral protein (ZAP) or Zinc finger CCCH-type antiviral protein 1 is a protein that in humans is encoded by the ZC3HAV1 gene. [5] [6] [7]This gene encodes a CCCH-type zinc finger protein that is thought to prevent infection by viruses by targeting viral RNA for degradation, inhibiting its translation as well as affecting programmed viral frameshifting.
Gene editing on TILs by genetic editing tools such as Zinc finger nucleases to decrease PD-1 expression on TILs is another way to improve the efficacy of TIL therapy. [1] Another major challenge to tackle is to reduce the 'on target, off tumor' toxicity of TCR therapy.
The restriction enzymes can be introduced into cells, for use in gene editing or for genome editing in situ, a technique known as genome editing with engineered nucleases. Alongside zinc finger nucleases and CRISPR/Cas9, TALEN is a prominent tool in the field of genome editing.
Examples of gene editing are CRISPR, zinc finger nuclease, transcription activator-like effector nuclease (TALEN), oligonucleotide directed mutagenesis + meganucleases. Genome editing, a type of genetic engineering; Gene therapy, the therapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease