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An endospore stain of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red Phase-bright endospores of Paenibacillus alvei imaged with phase-contrast microscopy An endospore is a dormant , tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota .
Endospores can last for decades in multiple hard conditions, such as drying and freezing. This is because the DNA inside the endospore can survive over a long period. Most bacteria are unable to form endospores due to their high resistance, but some common species are the genera Bacillus ( over 100 species) and Clostridium (over 160 species).
The wrong decision can be catastrophic: a vegetative cell will die if the conditions are too harsh, while bacteria forming spores in an environment which is conducive to vegetative growth will be out competed. [3] In short, initiation of sporulation is a very tightly regulated network with numerous checkpoints for efficient control. [citation ...
Endospores are bacterial survival structures that are highly resistant to many different types of chemical and environmental stresses and therefore enable the survival of bacteria in environments that would be lethal for these cells in their normal vegetative form.
Clostridium endospores have a distinct bowling pin or bottle shape, distinguishing them from other bacterial endospores, which are usually ovoid in shape. [ citation needed ] The Schaeffer–Fulton stain (0.5% malachite green in water) can be used to distinguish endospores of Bacillus and Clostridium from other microorganisms.
The term sporogenesis can also refer to endospore formation in bacteria, which allows the cells to survive unfavorable conditions. Endospores are not reproductive structures and their formation does not require cell fusion or division. Instead, they form through the production of an encapsulating spore coat within the spore-forming cell.
Clostridium sporogenes is a species of Gram-positive bacteria that belongs to the genus Clostridium. Like other strains of Clostridium, it is an anaerobic, rod-shaped bacterium that produces oval, subterminal endospores [2] and is commonly found in soil. Unlike Clostridium botulinum, it does not produce the botulinum neurotoxins.
Acid fast bacteria:Red Non acid fast: Blue 5 Endospore (Dornor's method) Primary stain Malachite green heat fixed to penetrate spores; vegetative cells are counterstained with Safranin Detects the presence of endospores in six genera of bacteria Endospores: Green Vegetative cells: Red 6 Capsule A: Hiss method (Positive technique)