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More than 50 years ago, MSI was introduced using secondary ion mass spectrometry (SIMS) to study semiconductor surfaces by Castaing and Slodzian. [5] However, it was the pioneering work of Richard Caprioli and colleagues in the late 1990s, demonstrating how matrix-assisted laser desorption/ionization (MALDI) could be applied to visualize large biomolecules (as proteins and lipids) in cells and ...
Mass spectrometry (MS) is an ... This oscillation generates an image current in the detector plates which is recorded by the instrument. The frequencies of these ...
Ion trajectories in an Orbitrap mass spectrometer. In mass spectrometry, Orbitrap is an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle.
For example, a sector instrument can be combined with a collision quadrupole and quadrupole mass analyzer to form a hybrid instrument. [7] A mass-selecting quadrupole and collision quadrupole with time-of-flight device as the second mass selection stage is a hybrid known as a quadrupole time-of-flight mass spectrometer (QTOF MS).
A triple quadrupole mass spectrometer (TQMS), is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a (non-mass-resolving) radio frequency (RF)–only quadrupole between them to act as a cell for collision-induced dissociation.
NanoSIMS (nanoscale secondary ion mass spectrometry) is an analytical instrument manufactured by CAMECA which operates on the principle of secondary ion mass spectrometry. [1] The NanoSIMS is used to acquire nanoscale resolution measurements [ 2 ] of the elemental and isotopic composition of a sample.
A microchannel plate within a Finnigan MAT 900 sector mass spectrometer position-and-time-resolved-ion-counting (PATRIC) scanning array detector. An external voltage divider is used to apply 100 volts to the acceleration optics (for electron detection), each MCP, the gap between the MCPs, the backside of the last MCP, and the collector .
Suitable image processing software can be used to import data from the mass spectrometer to allow visualization and comparison with the optical image of the sample. Recent work has also demonstrated the capacity to create three-dimensional molecular images using MALDI imaging technology and comparison of these image volumes to other imaging ...
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