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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).
The Wilhelmy plate consists of a thin plate usually on the order of a few square centimeters in area. The plate is often made from filter paper, glass or platinum which may be roughened to ensure complete wetting. In fact, the results of the experiment do not depend on the material used, as long as the material is wetted by the liquid. [1]
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
The sample is then cooled and inspected for pour point as per the usual pour point method. The method usually gives higher pour point because the thermal history has not been cancelled by a prolonged thermal treatment. The lower pour point is measured by first pouring the sample into a stainless steel pressure vessel. The vessel is then screwed ...
Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish or agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering.
The first residential building of slipform construction; erected in 1950 in Västertorp, Sweden, by AB Bygging Later picture of the residential building in Västertorp. Slip forming, continuous poured, continuously formed, or slipform construction is a construction method in which concrete is placed into a form that may be in continuous motion horizontally, or incrementally raised vertically.