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Blood culture bottle: Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms: Usually drawn first for minimal risk of contamination. [1] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms. [2] Blue ("light blue") Sodium citrate (weak calcium chelator ...
Blood is normally sterile. [1] The presence of bacteria in the blood is termed bacteremia, and the presence of fungi is called fungemia. [2] Minor damage to the skin [3] or mucous membranes, which can occur in situations like toothbrushing or defecation, [4] [5] can introduce bacteria into the bloodstream, but this bacteremia is normally transient and is rarely detected in cultures because the ...
Bacteremia is most commonly diagnosed by blood culture, in which a sample of blood drawn from the vein by needle puncture is allowed to incubate with a medium that promotes bacterial growth. [33] If bacteria are present in the bloodstream at the time the sample is obtained, the bacteria will multiply and can thereby be detected. [citation needed]
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Thus, the plate can be used either to estimate the concentration of organisms in a liquid culture or a suitable dilution of that culture using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms. Several methods are available to plate out cells. One technique is known as "streaking".
Duke lacrosse players wore No. 45 on their warmup jerseys in support of teammate Reade Seligmann before a March 2, 2007, game at College Park, Maryland.
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