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A bottle of iodine solution used on apples to determine the correct harvest time. The chart shows the level of residual starch. The cut surface of an apple stained with iodine, indicating a starch level of 4–5. The iodine–starch test is a chemical reaction that is used to test for the presence of starch or for iodine. The combination of ...
The iodine clock reaction is a classical chemical clock demonstration experiment to display chemical kinetics in action; it was discovered by Hans Heinrich Landolt in 1886. [1] The iodine clock reaction exists in several variations, which each involve iodine species ( iodide ion, free iodine, or iodate ion) and redox reagents in the presence of ...
The first step in the production of high-fructose corn syrup is the treatment of cornstarch with α-amylase, which cleaves the long starch polymers into shorter chains of oligosaccharides. An α-amylase called "Termamyl", sourced from Bacillus licheniformis, is also used in some detergents, especially dishwashing and starch-removing detergents ...
The Minor test (also known as Minor's test, the starch–iodine test, and the iodine–starch test), described by Victor Minor in 1928, [1] is a qualitative medical test that is used to evaluate sudomotor function (perspiration or sweating).
A redox titration [1] is a type of titration based on a redox reaction between the analyte and titrant. It may involve the use of a redox indicator and/or a potentiometer. A common example of a redox titration is the treatment of a solution of iodine with a reducing agent to produce iodide using a starch indicator to help detect the endpoint.
A common protocol used in the past for zymography of α-amylase activity was the so-called starch film protocol of W.W. Doane. Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or more properly, suspended) in it was overlaid for a period of time on top of the original gel. [6]
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As the reaction proceeds, all available SO 2 will be consumed and the starch indicator added to the solution will bind with the unconsumed iodine, turning the solution black. The second step of the reaction requires pretreating with solution with NaOH to release bound SO 2. The reaction with iodine can then be done. HSO 3 − ⇌H 2 SO 3 ⇌SO 2