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Lymphoblast. A lymphoblast is a modified naive lymphocyte with altered cell morphology. It occurs when the lymphocyte is activated by an antigen and increased in volume by nucleus and cytoplasm growth as well as new mRNA and protein synthesis.
The JY cell line is an Epstein–Barr virus (EBV)-immortalised B cell lymphoblastoid line. JY cells express HLA class-I A2 and class-II DR. JY is a suspension cell line, although the cells are known to grow in clumps. The growth medium is RPMI 1640, 10% fetal calf serum and 1% L-glutamine. [1] JY cells are positive for murine leukemia virus. [2]
Lymphoblastoid cell lines established from blood samples of humans who were centenarians (100 years old or older) have significantly higher PARP activity than cell lines from younger (20 to 70 years old) individuals, [13] again indicating a linkage between longevity and repair capability.
Lymphoblastoid cell line, the outcome of lymphocyte infection by Epstein–Barr virus; Light Center Length, the distance between the center of the filament (or arc tube) and a reference plane - usually the bottom of the lamp base
Raji cell culture. Raji is the first continuous human cell line of hematopoietic origin. [1] The Raji cell line is widely used as a transfection host. [2]Raji cells were derived from the B-lymphocytes of an 11-year-old Nigerian Burkitt lymphoma male patient in 1963 by R.J.V. Pulvertaft and was further worked on by B.O. Osunkoya (University College Hospital, Ibadan, Nigeria).
When EBV infects B cells in vitro, lymphoblastoid cell lines eventually emerge that are capable of indefinite growth. The growth transformation of these cell lines is the consequence of viral protein expression. [44] EBNA-2, EBNA-3C, and LMP-1, are essential for transformation, whereas EBNA-LP and the EBERs are not. [45]
Lymphoblastoid cell lines established from blood samples of humans who lived past 100 years (centenarians) were found to have a significantly higher activity of the DNA repair protein Poly (ADP-ribose) polymerase than cell lines from younger individuals (20 to 70 years old).
The life span of 13 mammalian species correlated with poly(ADP ribosyl)ation capability measured in mononuclear cells. Furthermore, lymphoblastoid cell lines from peripheral blood lymphocytes of humans over age 100 had a significantly higher poly(ADP-ribosyl)ation capability than control cell lines from younger individuals.