enow.com Web Search

  1. Ad

    related to: nitrocellulose membrane for western blot analysis

Search results

  1. Results from the WOW.Com Content Network
  2. Blot (biology) - Wikipedia

    en.wikipedia.org/wiki/Blot_(biology)

    Blotting Compass. In molecular biology and genetics, a blot is a method of transferring large biomolecules (proteins, DNA or RNA) onto a carrier, such as a membrane composed of nitrocellulose, polyvinylidene fluoride or nylon.

  3. Western blot - Wikipedia

    en.wikipedia.org/wiki/Western_blot

    The western blot method is composed of gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide, followed by an electrophoretic transfer onto a membrane (mostly PVDF or nitrocellulose) and an immunostaining procedure to visualize a certain protein on the blot membrane.

  4. Nitrocellulose - Wikipedia

    en.wikipedia.org/wiki/Nitrocellulose

    A nitrocellulose slide, nitrocellulose membrane, or nitrocellulose paper is a sticky membrane used for immobilizing nucleic acids in southern blots and northern blots. It is also used for immobilization of proteins in western blots and atomic force microscopy [15] for its nonspecific affinity for amino acids.

  5. Northwestern blot - Wikipedia

    en.wikipedia.org/wiki/Northwestern_blot

    A northern blot is a similar analytical technique that, instead of detecting a protein of interest, is used to study gene expression by detection of RNA (or isolated mRNA) on a similar membrane. The northwestern blot combines the two techniques, and specifically involves the identification of labeled RNA that interact with proteins that are ...

  6. Reverse phase protein lysate microarray - Wikipedia

    en.wikipedia.org/wiki/Reverse_phase_protein...

    Aliquots of the lysates are pooled and resolved by two-dimensional single lane SDS-PAGE followed by western blotting on a nitrocellulose membrane. The membrane is cut into four-millimeter strips, and each strip is probed with a different antibody. Strips with single band indicate specific antibodies that are suitable for RPMA use.

  7. Electroblotting - Wikipedia

    en.wikipedia.org/wiki/Electroblotting

    Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the following order from cathode to anode: sponge | three sheets of filter paper soaked in transfer buffer | gel | PVDF or nitrocellulose membrane | three sheets of filter paper soaked in transfer buffer | sponge.

  8. Polyacrylamide gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Polyacrylamide_gel...

    Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases ...

  9. Degradomics - Wikipedia

    en.wikipedia.org/wiki/Degradomics

    Capture antibodies spotted to nitrocellulose membranes can bind proteases in complex mixtures which have been pre-incubated and bound by detection antibodies allowing for parallel analysis of relative protease levels. [24] These arrays offer parallelization of protein levels over traditional western blot.

  1. Ad

    related to: nitrocellulose membrane for western blot analysis