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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
The plates are left upright on the bench to dry before inversion and incubation at 37 °C for 18 – 24 hours (or appropriate incubation conditions considering the organism and agar used). Each sector is observed for growth, high concentrations will give a confluent growth over the area of the drop, or a large number of small/merged colonies.
However, at that time, the agency was still existed on paper and physically inexistent. After procuring enough resources and assets, the agency finally came into being on 12 December 1975 thru Ministry of Health Decree No. 114/1975 and named as Badan Penelitian dan Pengembangan Kesehatan or colloquially abbreviated as Balitbangkes. [3]
The sample is then cooled and inspected for pour point as per the usual pour point method. The method usually gives higher pour point because the thermal history has not been cancelled by a prolonged thermal treatment. The lower pour point is measured by first pouring the sample into a stainless steel pressure vessel. The vessel is then screwed ...
Fractionation at total reflux. The Fenske equation in continuous fractional distillation is an equation used for calculating the minimum number of theoretical plates required for the separation of a binary feed stream by a fractionation column that is being operated at total reflux (i.e., which means that no overhead product distillate is being withdrawn from the column).
In non ideal fluid dynamics, the Hagen–Poiseuille equation, also known as the Hagen–Poiseuille law, Poiseuille law or Poiseuille equation, is a physical law that gives the pressure drop in an incompressible and Newtonian fluid in laminar flow flowing through a long cylindrical pipe of constant cross section.