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Compared to a volumetric pipette, a burette has similar precision if used to its full capacity, but as it is usually used to deliver less than its full capacity, a burette is slightly less precise than a pipette. [6] The burette is used to measure the volume of a dispensed substance, but is different from a graduated cylinder as its graduations ...
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
A graduated cylinder, also known as a measuring cylinder or mixing cylinder, is a common piece of laboratory equipment used to measure the volume of a liquid. It has a narrow cylindrical shape. Each marked line on the graduated cylinder represents the amount of liquid that has been measured.
French chemist François-Antoine-Henri Descroizilles developed the first burette (which was similar to a graduated cylinder) in 1791. [8] [9] [10] Gay-Lussac developed an improved version of the burette that included a side arm, and invented the terms "pipette" and "burette" in an 1824 paper on the standardization of indigo solutions. [11]
Pipette recalibration [17] is an important consideration in laboratories using these devices. It is the act of determining the accuracy of a measuring device by comparison with NIST traceable reference standards. Pipette calibration is essential to ensure that the instrument is working according to expectations and as per the defined regimes or ...
By means of a rubber tubing arrangement, the gas to be analyzed is drawn into the burette and flushed through several times. Using the stopcocks to isolate the absorption pipettes 100ml is typically withdrawn into the main burette for ease of calculation and the leveling flask is raised until the water is level between it and the burette.
In general, it is similar to a graduated cylinder, and is most commonly found in two sizes: 50 mL and 100 mL. It is closed at the top end with the bottom end immersed in water or mercury . The liquid traps a sample of gas in the cylinder, and the graduation allows the volume of the gas to be measured.
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