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To interact with host plant proteins many Agrobacterium virulence proteins encoded by vir genes. Agrobacterium vir gene expression occurs via the VirA-VirG sensor that results in generation of a mobile single-stranded T-DNA copy (T-strand). A processed form of VirB2 is the major component of the T-complex that is required for transformation.
Agrobacterium tumefaciens [3] [2] (also known as Rhizobium radiobacter) is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium . [ 4 ]
Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus.
A number of methods are available to transfer DNA into plant cells. Some vector-mediated methods are: Agrobacterium-mediated transformation is the easiest and most simple plant transformation. Plant tissue (often leaves) are cut into small pieces, e.g. 10x10mm, and soaked for ten minutes in a fluid containing suspended Agrobacterium. The ...
Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants.The most commonly used plant transformation vectors are T-DNA binary vectors and are often replicated in both E. coli, a common lab bacterium, and Agrobacterium tumefaciens, a plant-virulent bacterium used to insert the recombinant DNA into plants.
In plants the DNA is often inserted using Agrobacterium-mediated recombination, [21] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [22] Plant tissue are cut into small pieces and soaked in a fluid containing suspended Agrobacterium. The bacteria will attach to many of ...
Once inside the leaf the Agrobacterium remains in the intercellular space and transfers the gene of interest as part of the Ti plasmid-derived T-DNA in high copy numbers into the plant cells. The gene transfer occurs when the plant signals are induced and physical contact is made between the plant cells and the bacteria.
The T-DNA of Agrobacterium is approximately 15-20 kbp in length and will become integrated into the host plant genome upon its transfer via a process known as recombination. This process utilizes preexisting gaps in the host plant cell's genome to allow the T-DNA to pair with short sequences in the genome, priming the process of DNA ligation ...