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A number of methods are available to transfer DNA into plant cells. Some vector-mediated methods are: Agrobacterium-mediated transformation is the easiest and most simple plant transformation. Plant tissue (often leaves) are cut into small pieces, e.g. 10x10mm, and soaked for ten minutes in a fluid containing suspended Agrobacterium. The ...
The only essential parts of the T-DNA are its two small (25 base pair) border repeats, at least one of which is needed for plant transformation. [30] [31] The genes to be introduced into the plant are cloned into a plant transformation vector that contains the T-DNA region of the plasmid. An alternative method is agroinfiltration. [32] [33]
In research tobacco and Arabidopsis thaliana are the most frequently modified plants, due to well-developed transformation methods, easy propagation and well studied genomes. [63] [64] They serve as model organisms for other plant species. Introducing new genes into plants requires a promoter specific to the area where the gene is to be expressed.
Plant breeding can be performed using many different techniques, ranging from the selection of the most desirable plants for propagation, to methods that make use of knowledge of genetics and chromosomes, to more complex molecular techniques. Genes in a plant are what determine what type of qualitative or quantitative traits it will have.
Genetically modified plants have been engineered for scientific research, to create new colours in plants, deliver vaccines, and to create enhanced crops. Plant genomes can be engineered by physical methods or by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
In plants the DNA is often inserted using Agrobacterium-mediated transformation, [62] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [63] Other methods include biolistics, where particles of gold or tungsten are coated with DNA and then shot into young plant cells, [64 ...
Whilst the transformation efficiency is lower than in agrobacterial mediated transformation, which is also common in plant genetic engineering, particle bombardment is especially suitable for chloroplast transformation. Other transformation methods include the use of polyethylene glycol (PEG)- mediated transformation, which involves the removal ...