Search results
Results from the WOW.Com Content Network
Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Its production may be induced by a non-hydrolyzable analog of allolactose, IPTG, which binds and releases the lac repressor from the lac operator, thereby allowing the initiation of transcription to proceed. It is commonly used in molecular biology as a reporter marker to monitor gene expression.
IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to remove IPTG from the cells and therefore it is difficult to stop expression.
The lacUV5 promoter is a mutated promoter from the Escherichia coli lac operon which is used in molecular biology to drive gene expression on a plasmid. lacUV5 is very similar to the classical lac promoter, containing just 2 base pair mutations in the -10 hexamer region, compared to the lac promoter. [1]
The woman accusing Jay-Z and Sean “Diddy” Combs of sexually assaulting her when she was 13 can proceed anonymously (“at least for now”) in her lawsuit against the rappers, a judge ruled ...
50 Cent is mocking Jay-Z after a rape allegation was made against the "99 Problems" rapper. Jay-Z, whose legal name is Shawn Carter, 55, attended the premiere at the Dolby Theatre in Los Angeles ...
Expression of araB, araA and araC does not occur when arabinose is not present. In the absence of arabinose, AraC dimerizes while bound to the O 2 and I 1 operator sites, looping the DNA. The looping prevents binding of CAP and RNA Polymerase, which normally activate the transcription of both P BAD and P C .