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  2. Differential centrifugation - Wikipedia

    en.wikipedia.org/wiki/Differential_centrifugation

    Differential centrifugation, on the other hand, does not utilize a density gradient, and the centrifugation is taken in increasing speeds. The different centrifugation speeds often create separation into not more than two fractions, so the supernatant can be separated further in additional centrifugation steps.

  3. Centrifugation - Wikipedia

    en.wikipedia.org/wiki/Centrifugation

    Differential centrifugation is the simplest method of fractionation by centrifugation, [9] commonly used to separate organelles and membranes found in cells. Organelles generally differ from each other in density and in size, making the use of differential centrifugation, and centrifugation in general, possible.

  4. Density gradient - Wikipedia

    en.wikipedia.org/wiki/Density_gradient

    In the life sciences, a special technique called density gradient separation is used for isolating and purifying cells, viruses and subcellular particles. [5] Variations of this include Isopycnic centrifugation, Differential centrifugation, and Sucrose gradient centrifugation.

  5. Fractionation - Wikipedia

    en.wikipedia.org/wiki/Fractionation

    Differential centrifugation. Fractionation is a separation process in which a certain quantity of a mixture (of gasses, solids, liquids, enzymes, or isotopes, or a suspension) is divided during a phase transition, into a number of smaller quantities in which the composition varies according to a gradient.

  6. Buoyant density centrifugation - Wikipedia

    en.wikipedia.org/wiki/Buoyant_density_centrifugation

    The CsCl molecules become densely packed toward the bottom, so a continuous gradient of layers of different densities (and CsCl concentrations) form. Since the original solution was approximately the same density, they go to a level where their density and the CsCl density are the same, to which they form a sharp, distinctive band.

  7. Protein purification - Wikipedia

    en.wikipedia.org/wiki/Protein_purification

    Such an "equilibrium" centrifugation can allow extensive purification of a given particle. Sucrose gradient centrifugation—a linear concentration gradient of sugar (typically sucrose, glycerol, or a silica based density gradient media, like Percoll)—is generated in a tube such that the highest concentration is on the bottom and lowest on ...

  8. Isopycnic - Wikipedia

    en.wikipedia.org/wiki/Isopycnic

    Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this gradient is formed particles move within the gradient to the position having a density matching their own (this is in fact an incorrect description of the exact physical process but does describe the ...

  9. Category:Centrifugation - Wikipedia

    en.wikipedia.org/wiki/Category:Centrifugation

    Pages in category "Centrifugation" The following 8 pages are in this category, out of 8 total. ... Density gradient centrifugation; Differential centrifugation; E.