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  2. Alkaline lysis - Wikipedia

    en.wikipedia.org/wiki/Alkaline_lysis

    The steps of alkaline lysis can be summarized as the formation of a pellet, resuspension of the pellet in solution, cell lysis, neutralization, and centrifugation. [ 2 ] Alkaline lysis takes advantage of the small and supercoiled physical composition of plasmid DNA compared to chromosomal DNA, along with its ability to reanneal double stranded ...

  3. Plasmid preparation - Wikipedia

    en.wikipedia.org/wiki/Plasmid_preparation

    Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.

  4. Buffer P2 - Wikipedia

    en.wikipedia.org/wiki/Buffer_P2

    Buffer P2 is a lysis buffer solution produced by Qiagen.It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and 200mM NaOH.It is used in conjunction with other resuspension buffers and lysis buffers to release DNA from cells, often as part of the alkaline lysis method of purifying plasmid DNA from bacterial cell culture.

  5. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue.

  6. Lysis buffer - Wikipedia

    en.wikipedia.org/wiki/Lysis_buffer

    RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)

  7. AOL

    search.aol.com

    The search engine that helps you find exactly what you're looking for. Find the most relevant information, video, images, and answers from all across the Web.

  8. Agarose gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Agarose_gel_electrophoresis

    After the experiment is finished, the resulting gel can be stored in a plastic bag in a refrigerator. Electrophoresis is performed in buffer solutions to reduce pH changes due to the electric field, which is important because the charge of DNA and RNA depends on pH, but running for too long can exhaust the buffering capacity of the solution.

  9. Calcium chloride transformation - Wikipedia

    en.wikipedia.org/wiki/Calcium_Chloride...

    It enhances plasmid DNA incorporation by the bacterial cell, promoting genetic transformation. Plasmid DNA can attach to LPS by being added to the cell solution together with CaCl 2. [12] Thus, when heat shock is applied, the negatively charged DNA backbone and LPS combine, allowing plasmid DNA to enter the bacterial cell. [13]