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A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...
A comparison between the operations and effects of a cocurrent and a countercurrent flow exchange system is depicted by the upper and lower diagrams respectively. In both it is assumed (and indicated) that red has a higher value (e.g. of temperature) than blue and that the property being transported in the channels therefore flows from red to blue.
Countercurrent distribution, therefore, is a method of using a series of vessels (separatory funnels) to separate compounds by a sequence of liquid-liquid extraction operations. Contrary to liquid-liquid extraction, in the CCD instruments the upper phase is decanted from the lower phase once the phases have settled.
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [ 1 ] as well as in research and development.
Low-temperature ionic liquids (below 130 K) have been proposed as the fluid base for an extremely large diameter spinning liquid-mirror telescope to be based on the Moon. [25] Water is a common impurity in ionic liquids, as it can be absorbed from the atmosphere and influences the transport properties of RTILs, even at relatively low ...
The ability of the medium to swell in liquid is based on the cross-linking of these substances, the pH and the ion concentrations of the buffers used. [ 26 ] Incorporation of high temperature and pressure allows a significant increase in the efficiency of ion chromatography, along with a decrease in time.
The process was termed "counter-current liquid-liquid extraction." [5] Martin and Synge described the theory of this technique in reference to continuous fractional distillation described by Randall and Longtin. [6]
Salt metathesis is a common technique for exchanging counterions. The choice of reactants is guided by a solubility chart or lattice energy. HSAB theory can also be used to predict the products of a metathesis reaction. Salt metathesis is often employed to obtain salts that are soluble in organic solvents.