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A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [ 1 ] as well as in research and development.
Droplet countercurrent chromatography (DCCC or DCC) was introduced in 1970 by Tanimura, Pisano, Ito, and Bowman. [1] DCCC is considered to be a form of liquid-liquid separation, which includes countercurrent distribution and countercurrent chromatography, that employs a liquid stationary phase held in a collection of vertical glass columns connected in series.
Countercurrent chromatography (CCC) is a type of liquid-liquid chromatography, where both the stationary and mobile phases are liquids and the liquid stationary phase is held stagnant by a strong centrifugal force.
Countercurrent chromatography usually uses a planetary gear motion without rotary seals, while centrifugal partition chromatography uses circular rotation with rotary seals for liquid connection. CCC has interchanging mixing and settling zones in the coil tube, so atomization, extraction and settling are time and zone separated.
Countercurrent Chromatography is a method of separation, that is based on the differential partitioning of analytes between two immiscible liquids using countercurrent or cocurrent flow. [26] Evolving from Craig's Countercurrent Distribution (CCD), the most widely used term and abbreviation is CounterCurrent Chromatography (CCC), [ 27 ] in ...
Countercurrent distribution, therefore, is a method of using a series of vessels (separatory funnels) to separate compounds by a sequence of liquid-liquid extraction operations. Contrary to liquid-liquid extraction, in the CCD instruments the upper phase is decanted from the lower phase once the phases have settled.
Multicolumn countercurrent solvent gradient purification (MCSGP) is a form of chromatography that is used to separate or purify biomolecules from complex mixtures. It was developed at the Swiss Federal Institute of Technology Zürich by Aumann and Morbidelli. [1]