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Due to the extremely low amounts of liquids 0.1 - 10 nl and reagents, the methods to work with nanoliterplates are completely different compared to the methods used for microliter plates. Nanoliter plates reduce the amount of reagents, require lower sample volumes and increase the numbers of tests that can be performed in the lab.
The integrated nanoliter system consists of microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors. The microfabricated fluidic channels (basically very small pipes) act as the main transportation structures for any fluids as well as where reactions occur within the system.
This is necessary since the signal-to-noise ratio of sample sizes in the microliter to nanoliter range is dramatically reduced compared to bench-scale sample sizes, and microcoils have been shown to resolve this issue. [84] Mass spectrometry (MS) and high-performance liquid chromatography (HPLC) have also been used to overcome this challenge.
Microfluidic structures include micropneumatic systems, i.e. microsystems for the handling of off-chip fluids (liquid pumps, gas valves, etc.), and microfluidic structures for the on-chip handling of nanoliter (nl) and picoliter (pl) volumes. [68] To date, the most successful commercial application of microfluidics is the inkjet printhead. [69]
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