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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [ 1 ] [ full citation needed ] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [ 1 ]
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Preparation of microbiological samples in a laminar chamber. A laminar flow cabinet or tissue culture hood is a partially enclosed bench work surface designed to prevent contamination of biological samples, semiconductor wafer, or any particle-sensitive materials.
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
Indicator system: Pseudomonas aeruginosa, inoculated on to a nutrient agar plate is kept inside the jar along with the other plates. This bacteria need oxygen to grow ( aerobic ). A growth free culture plate at the end of the process indicates a successful anaerobiosis.
It is used when laboratory work requires a hotter flame than one attainable using a Bunsen burner, or when a flame of larger diameter is desired, such as when working with inoculation loop needing sterilization or in some glassblowing operations. The burner was introduced by French chemist Georges Méker in an article published in 1905.
[1] This table displays the steps involved in using a GasPak to create a completely anaerobic environment for incubating an inoculated agar plate. After inoculating the agar plate(s) with bacteria under aseptic conditions, the agar plates are placed in an anaerobic jar that contains components, like the catalyst chamber, that will help facilitate the reaction to eliminate free oxygen and ...