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  2. Variable pathlength cell - Wikipedia

    en.wikipedia.org/wiki/Variable_pathlength_cell

    Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.

  3. Langmuir adsorption model - Wikipedia

    en.wikipedia.org/wiki/Langmuir_adsorption_model

    The concentration of sites is given by dividing the total number of sites (S 0) covering the whole surface by the area of the adsorbent (a): [ S 0 ] = S 0 / a . {\displaystyle [S_{0}]=S_{0}/a.} We can then calculate the concentration of all sites by summing the concentration of free sites [ S ] and occupied sites:

  4. Area under the curve (pharmacokinetics) - Wikipedia

    en.wikipedia.org/wiki/Area_under_the_curve...

    In practice, the drug concentration is measured at certain discrete points in time and the trapezoidal rule is used to estimate AUC. In pharmacology, the area under the plot of plasma concentration of a drug versus time after dosage (called “area under the curve” or AUC) gives insight into the extent of exposure to a drug and its clearance ...

  5. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    In essence, the Beer Lambert Law makes it possible to relate the amount of light absorbed to the concentration of the absorbing molecule. The following absorbance units to nucleic acid concentration conversion factors are used to convert OD to concentration of unknown nucleic acid samples: [5] A260 dsDNA = 50 μg/mL A260 ssDNA = 33 μg/mL

  6. Schwarzschild's equation for radiative transfer - Wikipedia

    en.wikipedia.org/wiki/Schwarzschild's_equation...

    The absorption coefficient is fundamentally the product of a quantity of absorbers per unit volume, [cm −3], times an efficiency of absorption (area/absorber, [cm 2]). Several sources [ 2 ] [ 12 ] [ 3 ] replace nσ λ with k λ r , where k λ is the absorption coefficient per unit density and r is the density of the gas.

  7. Beer–Lambert law - Wikipedia

    en.wikipedia.org/wiki/Beer–Lambert_law

    The amount concentration c is then given by = (). For a more complicated example, consider a mixture in solution containing two species at amount concentrations c 1 and c 2 . The decadic attenuation coefficient at any wavelength λ is, given by μ 10 ( λ ) = ε 1 ( λ ) c 1 + ε 2 ( λ ) c 2 . {\displaystyle \mu _{10}(\lambda )=\varepsilon _{1 ...

  8. Absorbance - Wikipedia

    en.wikipedia.org/wiki/Absorbance

    Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". [1] Alternatively, for samples which scatter light, absorbance may be defined as "the negative logarithm of one minus absorptance, as measured on a uniform sample". [2]

  9. Molar absorption coefficient - Wikipedia

    en.wikipedia.org/wiki/Molar_absorption_coefficient

    The absorbance of a material that has only one absorbing species also depends on the pathlength and the concentration of the species, according to the Beer–Lambert law =, where ε is the molar absorption coefficient of that material; c is the molar concentration of those species; ℓ is the path length.