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Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. [1] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2]
Sucrose is a non-reducing sugar, so will not test positive with Benedict's solution. To test for sucrose, the sample is treated with sucrase. The sucrose is hydrolysed into glucose and fructose, with glucose being a reducing sugar, which in turn tests positive with Benedict's solution. [citation needed].
Aldonic acids are the products of the oxidation of aldoses by Benedict's or Fehling's reagents. [7] Copper ions react with an aldose to form a red precipitate, Cu 2 O. The reaction scheme of an aldose being oxidized by the copper ions in a Benedict's reagent solution. The R group provided is an example of a sugar backbone.
A reducing sugar is any sugar that is capable of acting as a reducing agent. [1] In an alkaline solution, a reducing sugar forms some aldehyde or ketone, which allows it to act as a reducing agent, for example in Benedict's reagent. In such a reaction, the sugar becomes a carboxylic acid.
Barfoed's test tests for reducing polysacchorides or disaccharides; Benedict's reagent tests for reducing sugars or aldehydes; Fehling's solution tests for reducing sugars or aldehydes, similar to Benedict's reagent; Molisch's test tests for carbohydrates; Nylander's test tests for reducing sugars; Rapid furfural test distinguishes between ...
All monosaccharide ketoses are reducing sugars, because they can tautomerize into aldoses via an enediol intermediate, and the resulting aldehyde group can be oxidised, for example in the Tollens' test or Benedict's test. [3] Ketoses that are bound into glycosides, for example in the case of the fructose moiety of sucrose, are nonreducing ...
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Several chemical tests utilize copper sulfate. It is used in Fehling's solution and Benedict's solution to test for reducing sugars, which reduce the soluble blue copper(II) sulfate to insoluble red copper(I) oxide. Copper(II) sulfate is also used in the Biuret reagent to test for proteins. Copper sulfate is used to test blood for anemia.