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  2. Diagnostic microbiology - Wikipedia

    en.wikipedia.org/wiki/Diagnostic_Microbiology

    The catalase test tests whether a microbe produces the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide. Smearing a colony sample onto a glass slide and adding a solution of hydrogen peroxide (3% H 2 O 2) will indicate whether the enzyme is present or not. Bubbling is a positive test while nothing happening is a negative ...

  3. Presumptive and confirmatory tests - Wikipedia

    en.wikipedia.org/wiki/Presumptive_and...

    The sample probably is the substance. For example, the Kastle–Meyer test will show either that a sample is not blood or that the sample is probably blood, but may be a less common substance. Further chemical tests are needed to prove that the substance is blood. Confirmatory tests are the tests required to confirm the analysis. Confirmatory ...

  4. Endospore staining - Wikipedia

    en.wikipedia.org/wiki/Endospore_staining

    Endospore stain on Bacillus subtilis.The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1]

  5. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    Gram staining is a bacteriological laboratory technique [8] used to differentiate bacterial species into two large groups (gram-positive and gram-negative) based on the physical properties of their cell walls. [9] [page needed] Gram staining can also be used to diagnose a fungal infection. [1]

  6. Spot analysis - Wikipedia

    en.wikipedia.org/wiki/Spot_analysis

    A spot assay or spot test can also refer to a specific test in microbiology. This test is often used to check the growth rate of bacterial or yeast cells on different media or to perform serial dilution tests of micro-organisms. Usually a 96-pinner (often called frogger) is used to perform these spot assay.

  7. Disk diffusion test - Wikipedia

    en.wikipedia.org/wiki/Disk_diffusion_test

    The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility ...

  8. Colonial morphology - Wikipedia

    en.wikipedia.org/wiki/Colonial_morphology

    Examining colonial morphology is the first step in the identification of an unknown microbe. The systematic assessment of the colonies' appearance, focusing on aspects like size, shape, colour, opacity, and consistency, provides clues to the identity of the organism, allowing microbiologists to select appropriate tests to provide a definitive ...

  9. Staining - Wikipedia

    en.wikipedia.org/wiki/Staining

    When starch is mixed with iodine in solution, an intensely dark blue colour develops, representing a starch/iodine complex. Starch is a substance common to most plant cells and so a weak iodine solution will stain starch present in the cells. Iodine is one component in the staining technique known as Gram staining, used in microbiology.