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So we find that the degree of rotation depends on the color of the light (the yellow sodium D line near 589 nm wavelength is commonly used for measurements) and is directly proportional to the path length through the substance and the amount of circular birefringence of the material which, for a solution, may be computed from the substance's ...
Within the machine the transducer that detects fluorescence created from the upper beam is located a distance away from the sample and at a 90-degree angle from the incident, upper beam. The machine is constructed like this to decrease the stray light from the upper beam that may strike the detector. The optimal angle is 90 degrees.
Some chemical substances are optically active, and linearly polarized (uni-directional) light will rotate either to the left (counter-clockwise) or right (clockwise) when passed through these substances. The amount by which the light is rotated is known as the angle of rotation.
The letters ASTC signify which of the trigonometric functions are positive, starting in the top right 1st quadrant and moving counterclockwise through quadrants 2 to 4. [5] Quadrant 1 (angles from 0 to 90 degrees, or 0 to π/2 radians): All trigonometric functions are positive in this quadrant.
In chemistry, specific rotation ([α]) is a property of a chiral chemical compound. [ 1 ] : 244 It is defined as the change in orientation of monochromatic plane-polarized light , per unit distance–concentration product, as the light passes through a sample of a compound in solution.
The binary degree, also known as the binary radian (or brad), is 1 / 256 turn. [21] The binary degree is used in computing so that an angle can be represented to the maximum possible precision in a single byte. Other measures of angle used in computing may be based on dividing one whole turn into 2 n equal parts for other values of n. [22]
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The orientation is usually chosen so that the 90-degree angle from the first axis to the second axis looks counter-clockwise when seen from the point (0, 0, 1); a convention that is commonly called the right-hand rule. The coordinate surfaces of the Cartesian coordinates (x, y, z). The z-axis is vertical and the x-axis is highlighted in green.