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Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology ...
Histologic specimen being placed on the stage of an optical microscope. Human lung tissue stained with hematoxylin and eosin as seen under a microscope.. Histology, [help 1] also known as microscopic anatomy or microanatomy, [1] is the branch of biology that studies the microscopic anatomy of biological tissues.
Movat's stain is a pentachrome stain originally developed by Henry Zoltan Movat (1923–1995), a Hungarian-Canadian Pathologist in Toronto [1] in 1955 to highlight the various constituents of connective tissue, especially cardiovascular tissue, by five colors in a single stained slide. [2]
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Anatomical terminology is used to describe microanatomical (or histological) structures. This helps describe precisely the structure, layout and position of an object, and minimises ambiguity. This helps describe precisely the structure, layout and position of an object, and minimises ambiguity.
The aim of staining is to reveal cellular components; counterstains are used to provide contrast. The most commonly used stain in histology is a combination of hematoxylin and eosin (often abbreviated H&E). Hematoxylin is used to stain nuclei blue, while eosin stains the cytoplasm and the extracellular connective tissue matrix of most cells ...
Masson's trichrome stain of rat airway. Connective tissue is stained blue, nuclei are stained dark red/purple, and cytoplasm is stained red/pink. Mouse skin stained with Masson's trichrome stain. Masson's trichrome is a three-colour staining procedure used in histology.
In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.